Track « Integrative Biology, Physiopathologies »

Proposal for a Master 2 internship – 2025-2026

Title 
Contribution of the satellite glial cells in PACAP-induced migraine-like symptoms

Summary :

PACAP (pituitary adenylate cyclase-activating polypeptide) has emerged as a key mediator in migraine, but its mechanism remains unknown. The aim of this study is to assess the contribution of the trigeminal satellite glial cells (SGCs) in PACAP-induced migraine.

Using behavioral testing, including both cephalic and extracephalic von Frey mechanical sensitivity assessments, along with measurement of intracellular cyclic adenosine monophosphate (cAMP) levels and calcium imaging in living cells of female rats, we will investigate the effects of chronic administration of PACAP38 on mechanical sensitivity changes. Furthermore, we will explore the potential impact of PACAP38 on both cAMP signaling and calcium dynamics in cultured satellite glial cells (SGCs) derived from the rat trigeminal ganglion. In parallel, we will examine the possibility of modulating SGC activity—pharmacologically or genetically—to determine their role in mediating PACAP38-induced changes in trigeminal nociceptive processing.

The project is being developed in collaboration with McGill University, Montreal, Canada

Methodologies (key words) : Intracellular cAMP measurement, Calcium imaging,  Behavioral testing, Pharmacological and/or genetic modulation of cell activity, immunohistochemistry

Labiratory : Neuro-Dol, Inserm/UCA 1107
Adress : 2 rue de Braga, 63000 Clermont-Ferrand, France
Contact:
Prof Radhouane DALLEL,
Neuro-Dol, Inserm/UCA U1107, Clermont-Ferrand
radhouane.dallel@uca.fr

Details here :